Journal: PLoS ONE

Article Title: Expression of the p53 Target Wig-1 Is Associated with HPV Status and Patient Survival in Cervical Carcinoma

doi: 10.1371/journal.pone.0111125

Figure Lengend Snippet: HPV status and chromosome 3 aberrations in the cell lines studied.

Article Snippet: The Saos2 cell line, originating from a human osteosarcoma was purchased from ATCC and used as a positive control for Wig-1 expression.

Techniques:

Journal: PLoS ONE

Article Title: Expression of the p53 Target Wig-1 Is Associated with HPV Status and Patient Survival in Cervical Carcinoma

doi: 10.1371/journal.pone.0111125

Figure Lengend Snippet: (A) CGH profiles of chromosome 3 in the eight cervical carcinoma cell lines and one control cell line (Saos2). Gains of chromosomal material are indicated by a bar to the right of the profile, and losses are marked to the left. A bold line indicates a region with amplification. The location of the probes for Southern analyses are shown next to the ideogram at the left. (B) Autoradiograms of Southern blot of cell line DNA after cleavage with EcoRI and hybridization with WIG-1 and subsequently with a central probe from 3p23. The names of the respective cell-lines are given above each lane. DNA from peripheral blood leukocytes (PBL) from a healthy donor was included as a normal reference. ‘+’ indicates relative gain of WIG-1 as compared to PBL. (C) Northern blot demonstrating WIG-1 mRNA levels in cervical carcinoma cell lines and the osteosarcoma cell line Saos2. Phosphorimage of Northern blot membrane hybridized with WIG-1 and subsequently with 18S as a loading control. Fibroblasts were included as reference. The two differently sized WIG-1 transcripts are indicated to the right. The expression levels were classified as negative (-), moderate (+) and strong (++). *Saos2 and **PBL were used as control cell lines.

Article Snippet: The Saos2 cell line, originating from a human osteosarcoma was purchased from ATCC and used as a positive control for Wig-1 expression.

Techniques: Control, Amplification, Southern Blot, Hybridization, Northern Blot, Membrane, Expressing

Journal: PLoS ONE

Article Title: Expression of the p53 Target Wig-1 Is Associated with HPV Status and Patient Survival in Cervical Carcinoma

doi: 10.1371/journal.pone.0111125

Figure Lengend Snippet: Copy number and expression of WIG-1/ Wig-1 in relation to HPV infection and TP53/p53 status.

Article Snippet: The Saos2 cell line, originating from a human osteosarcoma was purchased from ATCC and used as a positive control for Wig-1 expression.

Techniques: Expressing, Infection